Mechanical parameters were measured using cutometer with the cream added with Calendula officinalis. A significant improvement was measured in skin hydration and firmness. The functions and appearance of the skin depend on the water content of the stratum corneum and the balance of lipids on the skin surface. This balance varies depending on external factors such as humidity, UV radiation, temperature, and hormones. These mechanical properties of the skin are significantly affected by the epidermal hydration produced by the moisturizers. Calendula officinalis stimulates the epithelization and regeneration of injured skin. In the study, it was observed that the skin firmness was prevented, thus preventing the damage to the skin and slowing the aging process with the cream containing Calendula officinalis. Also, skin hydration was increased. This effect is very important for skin metabolism and also prevents skin changes and early aging.

Calendula officinalis contains phenolic acids, flavonoid glycosides (narcissin etc.), sterols, terpenoids, and triterpene alcohols. The effects of different concentrations of Calendula officinalis extract in in vitro human tissue cells were investigated. As a result of the study, it was observed that bioactive and free radical repellents contained in Calendula extract were protective against oxidative stress in human tissue cell model. Calendula has an antioxidant, anti-inflammatory, and antiviral effect.

The calendula officinalis family belongs to the Compositae family, its flowers are used and it contains antioxidants such as quercetin etc. It is a plant rich in terpenoids, carotenoids, flavonoids, and essential oils. Calendula officinalis extract (w/o) ensured skin firmness and increased skin elasticity. It also increased the skin hydration level and reduced transepidermal water loss (TEWL).